FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005279149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107813141
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1344420.12469908468764489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG438850.041.2415584
TCAGCGC538300.034.111375
CGATAAC212300.031.63653838-39
GCGATAA216050.031.2969636-37
ATAACCG220350.030.64215340-41
AGCGCGG474950.028.6909127
AGCGATA235000.028.64184636-37
TAACCGG239550.028.37454840-41
TAAGACG243750.027.795364-65
CAGCGCG543800.027.6262366
CGGAGTA267100.025.25959844-45
CGACCAA285050.023.83167570-71
CGCGGCG625950.021.9671699
ATCTCGA333600.020.55778918-19
TGCATCA905300.020.5430741
AACCGGA338400.020.40049742-43
AGACGAC345050.020.02251866-67
GCGCGGC706950.019.6717998
GAGTCGC349100.019.66141928-29
AGTCGCC372600.018.59347228-29
Produced by FastQC (version 0.11.5)