FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005279150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107813141
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG406450.033.952374
GCGATAA191500.027.90459336-37
CGATAAC186650.027.5868838-39
TCAGCGC520900.026.8690625
ATAACCG197600.026.12943640-41
TAACCGG211650.025.50578340-41
TAAGACG213200.024.98262264-65
AGCGATA214200.024.16015436-37
AGCGCGG451650.023.2598467
CGGAGTA238600.022.30192644-45
CAGCGCG533350.021.5311766
CGACCAA258850.020.07296470-71
ATCTCGA307400.017.68656218-19
CGCGGCG609050.017.5750649
AGACGAC318950.017.16864266-67
AACCGGA319100.016.55754942-43
GAGTCGC322950.016.3170628-29
AGTCGCC337050.016.29675928-29
TGCATCA867550.016.1665231
GCGCGGC693800.015.626718
Produced by FastQC (version 0.11.5)