FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57670443
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA81900.055.062084
TAGTCGA88900.050.357692
TCGATAT99350.045.7120555
CGATATG79500.042.067316
GTAGTCG114000.039.489551
AGTCGAT116050.038.8180963
TATGGCG79700.026.8217439
GCGATAA61800.018.13831736-37
CGATATT72050.017.7360746
CGATAAC61900.017.49476238-39
ATAACCG66050.016.46820840-41
TAACCGG72700.015.5498540-41
TAAGACG74950.015.46034564-65
AGCGATA72700.014.9614836-37
TTCGCGT45700.014.2401518-19
CGGAGTA81800.013.99305444-45
TCGCGTT47950.013.51960120-21
TACGCGC47150.013.44817838-39
GTACGCG49050.013.41171336-37
GTTCGCG49700.013.09406218-19
Produced by FastQC (version 0.11.5)