FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005279168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98675519
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGAAACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3187690.3230477054800188No Hit
CCTGGGAAACTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1081620.10961381414168189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGCG742050.042.798079
GGGAAAC1222750.038.3863834
GCGATAA422950.038.0028936-37
CGATAAC424800.036.95912638-39
GGAAACT1291550.036.466645
ATAACCG432650.036.3420640-41
AGCGATA442100.035.57246836-37
TAACCGG461900.034.94555740-41
TAAGACG461650.034.7980264-65
CGGAGTA470100.033.9444344-45
CGACCAA492550.031.9180570-71
ATCTCGA529850.030.53441618-19
GAGTCGC531700.029.63072628-29
AGTCGCC544050.029.5954128-29
AGACGAC547150.029.5509266-67
TTCGCGT189400.029.4264118-19
AACTGGC1107700.028.9577588
AACCGGA553550.028.58836742-43
TCGCGTT192800.028.51311120-21
GATAACC581900.028.03394738-39
Produced by FastQC (version 0.11.5)