FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005279169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119205165
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCCCAGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3093670.2595248284753433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA415350.037.49139836-37
CGATAAC417600.037.26717438-39
ATAACCG426450.036.6164240-41
AGGGGCG863050.036.3181469
TACCTCC1266800.036.245211
AGCGATA441550.035.30982236-37
TAACCGG449600.034.8208340-41
TAAGACG453000.034.5455964-65
CGGAGTA465300.033.2998444-45
CGACCAA486100.032.05907470-71
GAGTCGC533750.029.31811528-29
ATCTCGA538450.029.28179618-19
AGACGAC552300.028.50196666-67
AACCGGA550800.028.4920942-43
AGTCGCC555600.028.2249728-29
TTCGCGT179200.027.64500618-19
TCGCGTT183400.027.05076220-21
GTCGCCA579200.027.01258730-31
ACCGGAG608800.025.92590542-43
CGTCCAT610300.025.81916214-15
Produced by FastQC (version 0.11.5)