FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005279170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119205165
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCCCAGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2251580.1888827552061188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA337100.034.5066736-37
CGATAAC339500.032.98217438-39
ATAACCG351550.031.9382940-41
TAACCGG375500.031.19136840-41
AGCGATA363050.030.94124636-37
TAAGACG381000.030.60861664-65
TACCTCC1124600.029.3994081
CGGAGTA392900.029.38315244-45
AGGGGCG784500.028.6528859
CGACCAA416550.026.90248370-71
ATCTCGA457250.025.8058918-19
AGTCGCC471200.024.76654828-29
GAGTCGC457650.024.6332328-29
AGACGAC482300.024.32617866-67
TTCGCGT156600.024.1258118-19
AACCGGA479650.023.55648242-43
TCGCGTT162450.022.23358520-21
GTCGCCA508750.022.1956930-31
GATAACC539400.021.9170138-39
CGTCCAT526500.021.63144114-15
Produced by FastQC (version 0.11.5)