FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28093829
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGC37600.011.9993345
CGCCGTA42400.011.08994458-59
GCCGTAT45150.010.73006758-59
CGTATCA48050.010.03313360-61
TAACACG20300.09.1278992
CCGTATC55850.08.92955960-61
AAATCGG58950.08.5397164
GAAAAAT339100.07.95963141
CTCAAAT120050.07.785711
GTAGGGA737750.07.781524794-95
TGCGTCG50150.07.7686442
GTGTAGG1108350.07.69119594-95
CGGCGGT215750.07.68239748
GTAGGCT230550.07.62366694-95
TAGGCTA201150.07.604367794-95
ATCTAAC39350.07.6035646
TGTAGGG804550.07.58417594-95
CGTGTAG932400.07.4893294-95
TAGGGAA710900.07.443958894-95
AATCTAA73650.07.4156035
Produced by FastQC (version 0.11.5)