FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30451321
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG10300.019.70677233
CCCGTAT24650.013.3462261
TATGCCG23200.011.76933346
ACGCGAC18400.011.22214935
CGACCTA15550.011.0287624
AACGCGA19550.010.9197734
ATCGAAG53450.010.0183686
CGGAGAT54400.09.8432851
TCGTATG28300.09.77235943
GCGATAG24400.09.46682866
CGTATGC30050.09.43623244
CGCGTTA27000.09.33252434
CTCGTTT44700.09.2389371
TTACACG31000.09.14424932
TTCGCGC14550.09.1405817
CTCGTAT29350.09.06497142
TCCGATC20950.09.0209942
GTCGCTA26800.09.010324527
CTCGAAT42100.08.8119351
CGTATCG23200.08.74955753
Produced by FastQC (version 0.11.5)