FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27822836
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT40800.016.2396551
CGGGGTT98900.016.1462121
CTCGGGT78300.014.9759881
CCGGTCT100800.014.8960871
CCCGTAT29500.014.7042041
CGGGCAT102550.014.3629921
GCCGGTT61200.013.864071
CCGGGAT70200.013.5125751
GGCGGAT119450.013.3285371
GGCGGTT74250.012.9681221
CCCGGAT85700.012.9041471
GCCGGAT67300.012.89075851
CCCGGGT90350.012.8203611
CGGGGAT88950.012.8077861
CGGGAAT70700.012.742791
CCCGTTT71850.012.6051781
CGGCGGT59650.012.4662631
CCGGGTT84000.012.3140981
CGGGTGT72600.012.2123291
CTCGGTT74050.012.166311
Produced by FastQC (version 0.11.5)