FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27342721
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT4529431.6565395960409353Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC361690.1322801779676573Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA612650.040.24767758-59
CCGTATC621600.039.62283360-61
CGTATCA600550.038.36613560-61
GTATCAT653950.037.80389462-63
GCCGTAT610600.037.761458-59
ATCATTA687500.036.02821464-65
GTCGCCG706000.034.99680756-57
GGTCGCC663450.034.85091854-55
TCGCCGT667250.034.6730756-57
TGGTCGC728950.033.91523754-55
TCGGTGG757750.032.6104850-51
TATCATT711950.032.58662-63
GTGGTCG717300.032.31067352-53
GGTGGTC789650.031.43146952-53
CATTAAA794200.031.41988866-67
TCTCGGT792900.031.19660648-49
CGGTGGT748000.030.89879850-51
CTCGGTG789000.029.34021848-49
TCATTAA803200.028.95793364-65
GATCTCG861700.028.7746246-47
Produced by FastQC (version 0.11.5)