FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30073093
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT35100.031.5081921
CCCGATA49800.027.85521
GTCGGTT26850.025.9209691
CTCCCAT247950.024.6663651
CGCCTCA153650.022.62124
CCGATAC47200.022.467152
CCCGGAT81250.022.4121041
TCGGGTA29350.022.3591882
CCCGCCT156000.022.1612822
ACGCTTA59500.021.47183244-45
CTCCCCT214000.020.8276621
GTCCGGT32200.020.1337951
CTCCCTT318450.019.984011
ATGCACG64750.019.5111540-41
CCCGTTT67550.019.2654841
CCCAAAT449550.018.8855021
CCGTATC43400.018.8461992
CCGCCTC190850.018.6853263
CTCCTAT143900.018.6836171
TCGCCTA26750.018.6659722
Produced by FastQC (version 0.11.5)