FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23764077
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG192200.08.69976994-95
CGATAAT13903.6379788E-128.5761031
GCGTCGT286300.08.04709194-95
TCGTGTA214850.08.003715594-95
AATCCAA153150.07.9702085
CGGAAAT58650.07.96751551
CGAAAAT61350.07.85003571
CGTCGTG279600.07.75571994-95
AGCGTCG332600.07.75525494-95
CGCGTAT6150.0020778557.7533561
GTCGTGT244400.07.74543194-95
AATCCGC34050.07.6718335
ATCGGAA674000.07.643427494-95
GAATTGA136200.07.6020894
GATCGGA726700.07.57938694-95
GGAAAAT274800.07.5654471
GTGTAGG234850.07.514268494-95
CGCGAAT8952.7435648E-57.45881461
AAATCCA228800.07.30701834
CGACAAT33950.07.30345541
Produced by FastQC (version 0.11.5)