FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22315390
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTT51000.011.296451
GAGCGCG176150.011.1625886
AGAGCGC184650.010.6233755
AGCGCGG180850.010.5310837
GCGGTAT30900.010.3238831
GTCGGTT37300.09.9566341
CCGGCAT64050.09.8125421
GAGAGCG200350.09.7915334
GGCGGTT73000.09.587851
GGCGAAT39300.09.5710881
ACGTTCG19100.09.4492526
GGCGGAT108650.09.2027231
CTCGGTT69900.09.1956691
GCCGGTT71800.09.0186431
GCGCGGC220400.08.8999118
CGCGTAA15750.08.7668631
CCGGGTT97800.08.714471
GGGGAAT96000.08.3818951
CCGGTAT37500.08.3799111
CGACGGG157800.08.26152414-15
Produced by FastQC (version 0.11.5)