FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107578
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG25390.22923893396221304No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21540.19447840242402792No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG21420.1933949572851754No Hit
GTAATAAAGGGCCGTGTCTTCAGATCTCAGGCTGCTCAGTTCCATGTAGA20510.1851788316488771No Hit
ACCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG17740.1601693063603647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15600.14084786805082802No Hit
GGCTATAACTATGTCGACTGGTACTTGCAGAAGCCAGGGCACGCTCCGCA14750.13317346498395596No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12920.11665092661645501No Hit
CTTTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGACTGG12400.1119559976814274No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12330.11132398801709675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.10987939449862674No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11990.10825422679034795No Hit
GTCTACATGGAACTGAGCAGCCTGAGATCTGAAGACACGGCCCTTTATTA11900.10744164293620855No Hit
GGCCCTAGGTACTACTACTACTACGGTTTGGACGTCTGGGGCCAAGGGAC11880.10726106874639979No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCTTGCA11710.1057261881330254No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11630.10500389137379038No Hit
GTAATGGCTATAACTATGTCGACTGGTACTTGCAGAAGCCAGGGCACGCT11540.10419130751965099No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11120.10039924953366716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19200.075.154051
TCAACGC22150.063.758254
CAACGCA22550.062.9439665
ATCAACG22600.062.807553
GGTATCA7950.060.8038831
AACGCAG24000.059.085036
TATCAAC26950.053.20452
CGCAGAG29000.048.8935368
ACGCAGA31500.045.0131037
TACCGTC1601.986853E-631.4891787
GCAGAGT48400.030.3366819
GTACATG27900.029.2211861
ACCGTCG1000.0062941728.7901068
GTACACG7250.026.8324415
CAGAGTA34100.026.8060369
ACATGGG29050.026.787473
TACACGG7650.026.3462666
ATACCGT1959.2568935E-625.8396056
CGCAGTA1400.001036225425.7333322
CAGCGCT4900.023.5276154
Produced by FastQC (version 0.11.5)