FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107578
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG23380.21109122788643325No Hit
GTAATAAAGGGCCGTGTCTTCAGATCTCAGGCTGCTCAGTTCCATGTAGA21490.19402696694950605No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21460.19375610566479293No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG19460.17569868668391753No Hit
ACCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG15790.1425633228540112No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15260.1377781068240792No Hit
GGCTATAACTATGTCGACTGGTACTTGCAGAAGCCAGGGCACGCTCCGCA13960.1260407844865102No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12000.10834451388525232No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11780.10635819779735603No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCTTGCA11110.10030896243876278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14250.060.115191
TCAACGC15100.055.7172434
ATCAACG15550.054.10243
CAACGCA15550.053.6472635
AACGCAG17350.048.9216046
TATCAAC18600.045.2307742
GGTATCA6350.043.078641
TATACCG700.001099649141.0943345
CGCAGAG20650.040.7534458
ACGCAGA22250.037.824577
GTACATG30600.031.7588271
ACATGGG30000.029.4811843
GCAGCGC6400.028.0969098
CAGAGTA25700.026.0284529
CAGCGCT5850.025.8203379
TACACGG8000.024.2768256
GTACACG7550.023.8129395
GCAGAGT38600.022.7337479
GTCTAAC1600.002266991922.4958341
GGCAGCG8400.022.2644627
Produced by FastQC (version 0.11.5)