FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146252
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53700.25020361075959396No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC44430.20701203772902715No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT28500.13278962582213086No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27910.13004064760335693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.12486884112396866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.11815947055611364No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG24460.11396611395120425No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23610.11000572160212314No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA23210.10814200755549674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58350.082.9742361
GGTATCA23500.077.56531
ATCAACG74950.063.8898743
TCAACGC75700.063.1617624
CAACGCA82450.058.161455
AACGCAG85200.056.111236
TATCAAC85600.056.1092032
CGCAGAG89800.053.2369358
GCAGAGT101600.047.1956379
ACGCAGA107550.044.5176857
CAGCGCT14050.032.2838679
GTACATG82650.030.7713581
TACATGG81800.030.282182
ACATGGG82100.029.732993
CAGAGTA101000.028.2289759
AGCGCTG13800.028.1751585
CATGGGG49500.026.4757464
GTATACG2207.9141137E-726.1988451
GTACACG25050.025.8694065
TACACGG25250.025.3775636
Produced by FastQC (version 0.11.5)