FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101945
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA42830.38867638584502856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT41420.375880828897994No Hit
ATATTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAA38180.3464782725090635No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33080.300196470785747No Hit
GTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAG29140.26444151023871426No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCT24310.22060992154780862No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21110.1915703596821983No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG20130.18267699386085515No Hit
GTTTTGTACAGCTCCGACAAGAACTACTTAGCTTGGTACCAGCAGAAACC19760.17931929452014395No Hit
GTATATTACTGTGCGAGCCAGGTATATTGTTCTGGTGGTGACTGCCGACC18260.16570699989563906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17880.16225855192409785No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT17660.16026208204583714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17340.1573581258592761No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16990.15418192378022497No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16500.14973524086955337No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16470.1494629949770633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16160.14664978742133228No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15850.14383657986560128No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15480.14047888052489008No Hit
GTTTACATGGAGGTGAGCAGCCTCAGATCTGACGACACGGCCGTATATTA15090.13693968392251882No Hit
GTAATATACGGCCGTGTCGTCAGATCTGAGGCTGCTCACCTCCATGTAAA15040.13648594076836867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14550.13203925785769707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14180.12868155851698587No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14170.12859080988615584No Hit
CTCCTAAGCTCCTCATTTACTGGGCATCTACGCGGGAATCCGGGGTCCCT14040.12741107768536541No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14000.1270480831620453No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13650.12387188108299416No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13590.12332738929801397No Hit
GTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA13500.12251065162054368No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12690.11516001252331105No Hit
CCTCATTTACTGGGCATCTACGCGGGAATCCGGGGTCCCTGACCGATTCA12070.10953359741184905No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11970.10862611110354872No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11770.10681113848694808No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11720.10635739533279792No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11690.10608514944030782No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11350.10299969599208672No Hit
TTCCAGGACAGAGTCACGGTTACCGCGGACGAATCCACGAGCACTGTTTA11300.10254595283793655No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11180.10145696926797618No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11080.10054948295967585No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11050.10027723706718575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17600.076.6375351
GGTATCA6250.068.0901951
ATCAACG20750.064.293573
TCAACGC20950.063.6797874
CAACGCA21800.061.194085
TATCAAC22300.059.8247342
AACGCAG24150.055.1641436
CGCAGAG27800.048.160738
ACGCAGA33400.039.870367
GCAGAGT33350.039.4984639
CAGCGCT6500.037.652219
TTTCGCG1001.451464E-435.991089
TAAACCG850.00281562133.9340135
GTACATG28300.033.6434171
ACATGGG29050.032.767383
TATACGG8400.032.621095
ATACGGC8800.031.0959076
GCAGCGC8250.030.537898
TACGGCC9150.029.1075427
CAGAGTA31850.028.7024659
Produced by FastQC (version 0.11.5)