FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101945
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA41290.3747010966972036No Hit
ATATTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAA38070.3454800375699332No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37090.33658667174859No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30110.27324412742922743No Hit
GTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAG29230.2652582479161846No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCT22510.2042751679984028No Hit
GTATATTACTGTGCGAGCCAGGTATATTGTTCTGGTGGTGACTGCCGACC22040.20000998234939132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18840.17097042048378094No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18170.16489026221816877No Hit
GTTTTGTACAGCTCCGACAAGAACTACTTAGCTTGGTACCAGCAGAAACC17790.16144181424662757No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17500.15881010395255662No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16840.15282069431777448No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16490.14964449223872334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16300.14792026825295274No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15960.14483481480473162No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15540.14102337230987028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14940.13557845446006833No Hit
GTTTACATGGAGGTGAGCAGCCTCAGATCTGACGACACGGCCGTATATTA14860.13485246541342807No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14280.1295890448252862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13730.12459787012963441No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13200.1197881926956427No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12920.1172472310324018No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12860.11670273924742161No Hit
CTCCTAAGCTCCTCATTTACTGGGCATCTACGCGGGAATCCGGGGTCCCT12650.11479701799999092No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12480.11325429127588037No Hit
GTAATATACGGCCGTGTCGTCAGATCTGAGGCTGCTCACCTCCATGTAAA12400.11252830222924011No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12360.11216530770592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12290.11153006729010975No Hit
GTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12110.10989659193516917No Hit
GGTATATTGTTCTGGTGGTGACTGCCGACCTTTTGACTTCTGGGGCCAGG11560.1049054172395174No Hit
GCCGTATATTACTGTGCGAGCCAGGTATATTGTTCTGGTGGTGACTGCCG11440.103816433669557No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11310.10263670146876658No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11130.101003226113826No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG11060.10036798569801578No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11050.10027723706718575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.067.094351
ATCAACG15350.057.1548583
TCAACGC15300.056.8716244
TATCAAC16650.053.5585632
CAACGCA16950.051.759715
CGCAGAG18050.049.057358
AACGCAG18450.048.309166
GGTATCA5550.047.95011
GCAGAGT22550.039.9043129
ACGCAGA22900.038.9818467
GTACATG29400.038.1643641
ACATGGG30750.035.5467723
TATACGG7650.033.840975
TGTACGA1102.5548963E-432.6887862
ATACGGC7900.031.8452846
CATGGGA15800.030.9494934
TACGGCC8300.030.357517
CAGCGCT7750.029.7238249
GTACACG11400.029.0171455
GTACTAT20600.028.2812461
Produced by FastQC (version 0.11.5)