FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070266
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATGGCCGTGTCCTCAGATCTCAGGCTGTGCAGTTCCAGGAAGA49160.4593250649838451No Hit
ATCTATTACTGTGCGAGAGGTTCCCACCCAGTGGCAACTACCCCCTTTAA43930.41045870839585674No Hit
ATATTAGTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA30820.28796579541908274No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25920.2421827844666653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20850.19481138333834766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20190.18864469206720572No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19860.18556134643163477No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19780.1848138687017994No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19620.1833189132421286No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18490.17276079030820374No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17520.1636976228339497No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17510.16360418811772026No Hit
GACTAATATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG17270.1613617549282141No Hit
TCCTGGAACTGCACAGCCTGAGATCTGAGGACACGGCCATCTATTACTGT16670.1557556719544487No Hit
CATTATATTAGTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA16520.15435415121100735No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCC16340.15267232631887775No Hit
AATATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGC15950.14902837238593022No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15090.1409929867901998No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG15060.14071268264151154No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT14700.1373490328572523No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCGTCGGTGAAGATCTCTTGTA14440.1349197302352873No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGCTGGCAGCAG13830.12922021254529248No Hit
GCCTGAGATCTGAGGACACGGCCATCTATTACTGTGCGAGAGGTTCCCAC13580.1268843446395569No Hit
GTCACGATGAGTGCGGACGAATCCACGACCACAGTCTTCCTGGAACTGCA13500.1261368669097215No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13340.12464191145005073No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13210.12342726013906823No Hit
CTATTACTGTGCGAGAGGTTCCCACCCAGTGGCAACTACCCCCTTTAAGT12860.1201570450710384No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG12670.11838178546267938No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG12550.11726056886792628No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12440.11623278698940263No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12430.11613935227317322No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11990.11202822475907857No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11810.11034639986694895No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11800.11025296515071956No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA11550.10791709724498395No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11430.10679588065023089No Hit
CAGTAATAGATGGCCGTGTCCTCAGATCTCAGGCTGTGCAGTTCCAGGAA11170.10436657802826586No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11140.10408627387957758No Hit
CATCGTGACTGTGCCCTGGAATTTCTGTGCGTAGTTGCCTGTGCCAAAGG11100.1037125350146599No Hit
TCTATTACTGTGCGAGAGGTTCCCACCCAGTGGCAACTACCCCCTTTAAG10980.10259131841990683No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10980.10259131841990683No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10720.10016201579794182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17350.073.9728241
ATCAACG20000.064.144413
TCAACGC20400.062.5333754
CAACGCA20950.060.8888445
AACGCAG21800.058.1216136
TATCAAC22300.057.5286182
CGCAGAG26700.047.446238
ACGCAGA30650.041.3316277
GCAGAGT31400.040.5736359
ATGGCCG23250.034.363738
AATAGAT24100.034.0923233
GTAATAG24350.034.052621
TAATAGA24600.033.106412
ATAGATG24950.032.9308624
TAGATGG25950.032.4935345
TGGCCGT24750.032.2810829
GTACATG29100.031.467511
TAGTCCG19150.031.236175
ACATGGG28950.030.8703753
GTCTAGA2351.3387762E-930.682011
Produced by FastQC (version 0.11.5)