FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070266
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATGGCCGTGTCCTCAGATCTCAGGCTGTGCAGTTCCAGGAAGA46410.4336305180207537No Hit
ATCTATTACTGTGCGAGAGGTTCCCACCCAGTGGCAACTACCCCCTTTAA39730.3712161275794989No Hit
ATATTAGTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA27970.2613369012936971No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23740.22181401632865103No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21440.20032403159588363No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18700.17472291934902162No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18610.17388200690295683No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18480.17266735559197433No Hit
GACTAATATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG17850.16678096846952067No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17550.16397792698263797No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17530.16379105755017911No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17050.1593061911711668No Hit
CATTATATTAGTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA16270.15201828330527176No Hit
AATATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGC16200.1513642402916658No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15890.1484677640885537No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCC14760.13790964115462886No Hit
TCCTGGAACTGCACAGCCTGAGATCTGAGGACACGGCCATCTATTACTGT14590.13632125097872866No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCGTCGGTGAAGATCTCTTGTA13690.12791212651808054No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13360.12482878088250957No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGCTGGCAGCAG12910.12062421865218555No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12360.11548530925956724No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12340.1152984398271084No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12170.11371004965120822No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11920.11137418174547262No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11910.11128074702924319No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT11870.11090700816432551No Hit
GCCTGAGATCTGAGGACACGGCCATCTATTACTGTGCGAGAGGTTCCCAC11670.10903831383973703No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11660.10894487912350762No Hit
CTATTACTGTGCGAGAGGTTCCCACCCAGTGGCAACTACCCCCTTTAAGT11590.10829083610990164No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG11320.10576809877170723No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11260.10520749047433069No Hit
GTCACGATGAGTGCGGACGAATCCACGACCACAGTCTTCCTGGAACTGCA11130.10399283916334819No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10790.10081605881154779No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10770.10062918937908892No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG10730.10025545051417124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)