FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767176
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCATCTGCCTGGGCCCCCCTGATGGTGAGGGCAG30080.3920873437125249No Hit
GATTATTACTGTGCGCTTTATGTTGCAGTTGATATTTGGGCGTTCGGCGG26740.3485510495635943No Hit
GTAATACATGGCCGTGTCTTCTACTCGCAGGCTGTTCATTTGTAGATAAA15790.20581978581186064No Hit
TTATTACTGTGCGCTTTATGTTGCAGTTGATATTTGGGCGTTCGGCGGAG11630.15159494040480934No Hit
ATGTATTACTGTGCGAAAGATTACGCCCGCTCGAGTTATTATACTGGTAA10970.1429919601238829No Hit
CAGTAATAATCAGCCTCATCATCTGCCTGGGCCCCCCTGATGGTGAGGGC10280.13399793528473258No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10070.1312606233771651No Hit
GTGGTGACCCAGGAGCCATCGCTCTCAGTGACCCCTGGAGGGACAGTCAC10020.1306088824467919No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGCTCTCAGTGACCCCTGGAG9690.12630739230632865No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9470.12343973221268653No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9240.12044172393296974No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9130.11900789388614869No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT8200.10688551258120692No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7690.10023775509140015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9050.058.9595344
GTATCAA9650.057.5541041
ATCAACG9450.056.4638943
CAACGCA10000.053.354895
AACGCAG10400.051.2424776
CGCAGAG11950.044.5988778
ACTTGCG750.001532258238.4112858
GGTATCA5250.037.0951731
GTAATAC10900.037.0573581
GCAGAGT14550.036.631719
ATGGCCG10250.033.7269828
TGGCCGT10650.032.462369
GTACACG3200.031.5442755
CTGTGCG21450.031.2279979
GTACATG19500.031.0711691
AATCAGC15750.030.635576
ATCAGCC15600.030.470497
ACATGGG18700.030.4618593
TAATCAG16250.029.727885
TTATTAC18950.028.5379643
Produced by FastQC (version 0.11.5)