FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785344
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGAACA47260.26471089045024376No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35160.19693683682248353No Hit
GTATTGGAGCAGTCACCAGTGGTTATGTTCCAAACTGGTTCCAGCAGAAA28760.16108940349870948No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT27750.1554322304273014No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACGCCCAAGCACCACCA26680.14943898766848293No Hit
GTGGTGACTCAGGAGCCCTCACTGACGGTGTCCCCAGGAGGGACAGTCAC26310.14736655792945225No Hit
ATTTATTACTGTGCGAGAGAATTTAATGGTGGCCACTTTGACTACTGGGG26220.14686245339833667No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG23690.13269151491253225No Hit
ACTATATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG23470.13145925939202752No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23300.1305070619443648No Hit
GATTTATAGCACAAGCAACAAACACCCCTGGACCCCTGCCCGGTTCTCAG23000.12882671350731287No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG21520.12053699455119014No Hit
CCCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG21510.12048098293662174No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21410.11992086679093777No Hit
CTACTATATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA21090.11812849512474906No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACGCCCA20340.11392762403211928No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19920.11157513622024663No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA19890.11140710137654145No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19160.10731825351304847No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18990.10636605606538573No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18790.10524582377401778No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18120.10149304559793519No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACGGTGTCCCCAGGAG18090.10132501075423No Hit
GTATTACTGCCTGCTCTACCATGGTGGTGCTTGGGCGTTCGGCGGAGGGA18020.10093292945225123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29600.065.507781
ATCAACG32550.058.6800843
TCAACGC33000.057.8798944
TATCAAC35150.054.3395842
CAACGCA34900.054.314275
GGTATCA12600.052.6319731
AACGCAG36850.051.6313556
ACGCAGA40250.047.2487567
CGCAGAG40850.046.538
GTACATG46850.036.772291
GTACACG10350.035.5151335
TACATGG47650.035.2442932
TACACGG10550.034.8389326
ACATGGG47950.034.2722053
ATGGCCG25850.032.587168
TACACCG2456.002665E-1132.360135
ACACGGG12750.029.9445677
AATGGCC28250.029.8345667
GTATGTA16050.029.1924571
TATGTAC14050.027.7021352
Produced by FastQC (version 0.11.5)