FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785344
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGAACA46560.260790077430456No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC34760.19469637223974764No Hit
GTATTGGAGCAGTCACCAGTGGTTATGTTCCAAACTGGTTCCAGCAGAAA30210.16921108761112705No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT26270.14714251147117866No Hit
ATTTATTACTGTGCGAGAGAATTTAATGGTGGCCACTTTGACTACTGGGG26220.14686245339833667No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACGCCCAAGCACCACCA25300.14170938485804419No Hit
GTGGTGACTCAGGAGCCCTCACTGACGGTGTCCCCAGGAGGGACAGTCAC24130.1351560259535417No Hit
CCCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG23030.12899474835101807No Hit
GATTTATAGCACAAGCAACAAACACCCCTGGACCCCTGCCCGGTTCTCAG22980.12871469027817609No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22650.126866306997419No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG22590.1265302373100086No Hit
ACTATATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG22370.12529798178950388No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21150.11846456481215946No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACGCCCA20630.1155519608546028No Hit
CTACTATATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA20460.11459976340694007No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19480.10911062517923716No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG19270.10793438127330082No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18660.10451767278462862No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18640.10440564955549184No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA18490.10356547533696588No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18330.10266928950387152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24350.061.9216881
TCAACGC25200.057.5088654
ATCAACG25550.057.2870673
CAACGCA28300.051.21655
TATCAAC29850.049.0360372
AACGCAG29700.048.742026
GGTATCA10900.047.6542321
ACGCAGA31100.046.5491457
CGCAGAG33100.043.5189138
GTACGGT1051.9088351E-434.353911
GTACATG49300.031.4620481
TACATGG50750.030.2626672
ACATGGG51050.029.6602653
TATTGAG25350.028.161615
TTATTGA26000.026.6217824
ATGGCCG25100.025.538328
CATGGGG31250.024.2258224
GCAGAGT62700.023.204499
ATTGAGT18000.022.4067766
TCTACAC11950.022.324683
Produced by FastQC (version 0.11.5)