FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206551
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAAC36830.30525025465148176No Hit
CTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCT32120.2662133635461742No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27590.22866832815189742No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21110.17496152255478634No Hit
ACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGC16380.13575886970380863No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14550.12059166997499483No Hit
CAGTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAA14370.11909981426396399No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13750.113961200148191No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13730.1137954384025209No Hit
GAATTCACACTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA13370.11081172698045917No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12460.10326956755246981No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG12270.10169483096860389No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12100.10028585613040808No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)