FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853153
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.14839573418924396No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCACCGTCAGGGAGA22670.12233204705709673No Hit
GTGTATTACTGTGCGAGTTCGTCAGTTACTGGCACCAGGGTGGGTCTACT19870.10722266321237371No Hit
GTCTCTGGTGACTCCATCAATAGTCCTAGTTGGTACTGGGTCTGGCTCCG19270.10398493810279022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.066.2602841
GTATCAA36450.066.2016451
TCAACGC40000.059.5834124
ATCAACG42200.056.4771733
CAACGCA42300.056.3421365
AACGCAG42850.055.6189586
TATCAAC47500.051.2352372
CGCAGAG54050.043.9594468
ACGCAGA56600.042.1061487
GCAGAGT65100.037.1614159
GTACATG53650.029.671811
TTAGCGC1000.0062829328.8016513
ACATGGG54400.027.266273
TACATGG55700.027.016982
CAGAGTA61350.025.349729
GTCTAGG6300.021.7237381
GTAATAC19000.021.230291
CATGGGA27150.019.8906444
GTATTAG9950.019.5461941
TACACGG7950.019.0194456
Produced by FastQC (version 0.11.5)