FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853153
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.1393840659675699No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCACCGTCAGGGAGA21050.11359018926122127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34650.069.8160861
TCAACGC39650.059.5658044
GGTATCA16550.059.1643941
ATCAACG41200.057.324863
CAACGCA41850.056.4329835
AACGCAG43300.054.5461436
TATCAAC46000.052.12582
CGCAGAG52900.044.6474088
ACGCAGA53450.044.187997
GCAGAGT61100.038.6481489
GTATACG1352.2474842E-531.999041
GTACATG52050.026.9732571
CAGAGTA57550.026.0204059
TACATGG54800.025.3596422
ACATGGG54200.024.7104133
GTAATAC17850.023.7976071
TACACGG8200.023.7098056
ACAGCCG30450.022.2289338
CAGCCGT19750.021.5070559
TACACAG30850.021.2393325
Produced by FastQC (version 0.11.5)