FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722519
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGAGAGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC41350.5723032889100494No Hit
CTCCAATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCC30170.4175668736739103No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29700.4110618544287416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24630.34089068937979483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17010.2354263348091884No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16890.23376547883169851No Hit
CTTCTAGGTTCAGCGGCAGTGGATCTGAGACAGAATTCACTCTCACCATC15790.2185409657047081No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15710.21743372838638153No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14360.19874909863962054No Hit
GGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGC13040.18047968288723204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12580.17411306830685422No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12210.1689920957095938No Hit
ACTTATTACTGTCAACAGTATTGGAGAGGGTGGACGTTCGGCCAAGGGAC11910.16483995576586913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11230.15542843856009322No Hit
ACCTAGAAGGGACCCCACTTTGTAAAAAAGACGCCTTATAGATCAGGAGT11210.1551516292305116No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11120.15390598724739418No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10960.15169151261074104No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10810.14961544263887872No Hit
TCTATAAGGCGTCTTTTTTACAAAGTGGGGTCCCTTCTAGGTTCAGCGGC10680.14781618199659802No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10220.1414495674162202No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10170.1407575440922661No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9940.13757423680207717No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9820.13591338082458732No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9740.13480614350626072No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9700.13425252484709746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9420.13037719423295444No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9390.12996198023858196No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8900.12318015166383169No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGATGGTGAGAGTGA8780.1215192956863418No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8630.11944322571447949No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8620.11930482104968866No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8600.11902801172010702No Hit
ATTGGAGAGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA8600.11902801172010702No Hit
GTCTCTGGTGACGCCATCAGTCGTGAGTTGTGGAGCTGGATCCGGCAGCC8490.11750556040740798No Hit
TTATTACTGTCAACAGTATTGGAGAGGGTGGACGTTCGGCCAAGGGACCA8270.1144606577820099No Hit
GAATTCACTCTCACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTA8210.11363022979326495No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG7950.11003170850870357No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC7910.10947808984954029No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7860.10878606652558619No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7860.10878606652558619No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7810.10809404320163206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG7590.10504914057623398No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7420.10269626127479001No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7360.10186583328604508No Hit
AGTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7280.1007585959677185No Hit
CACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCAAC7260.10048178663813684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12900.052.5708663
TCAACGC12900.052.5708664
TATCAAC14000.048.959022
CAACGCA14000.047.9249765
GAATACG1700.046.610929
AACGCAG14650.046.2204486
CGCAGAG15050.044.0347188
GCAGAGT17950.037.321789
ATAGGCC1301.7106267E-533.2977523
CCAATAC18250.032.0205763
CAATACT18700.031.6358324
TCCAATA19800.030.6091462
ACTGTTG20050.030.1792778
GTACATG21500.029.8834061
ATACTGT21450.029.5528456
TACTGTT20850.029.366817
TACATGG21900.028.3329322
AATACTG21450.028.2526365
ACATGGG22000.027.8742523
GTGCTAG3153.6379788E-1127.5010451
Produced by FastQC (version 0.11.5)