FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492474
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGAAACA12750.25889691638543355No Hit
GTTTATTACTGTGCGGCCAACCACCTGCCATGGGCTTTTGATTTCTGGGG7860.15960233433643195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.14620061160589187No Hit
GCATAATATGTGTTAGTGCTAGTACTACCGCTATAAGTAATTGAGACCCA6880.13970280664563No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6450.13097138123027816No Hit
ATATTATGCAGACTCCGTGGAGGGCCGGTTTACCATCTCCAGAGATAATT6310.12812859156016357No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6050.12284912502995082No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA5900.11980327895482806No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC5810.11797577130975441No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT5640.11452381242461532No Hit
ATTATATATCGTGGTATCAGCAGCTCCCTGGAACAGCCCCCAGACTCCTC5530.1122901919695253No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA5480.11127490994448437No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT5400.10965045870441892No Hit
AACACATATTATGCAGACTCCGTGGAGGGCCGGTTTACCATCTCCAGAGA5320.10802600746435344No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG5270.10701072543931253No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC5220.10599544341427163No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5160.10477710498422252No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5100.10355876655417343No Hit
ATATGTGTTAGTGCTAGTACTACCGCTATAAGTAATTGAGACCCACTCCA5030.10213737171911613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)