FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944190
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAATGGTGCTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTA36400.3855156271513149No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35710.3782077759772927No Hit
CCATTAAAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGC33630.35617831156864616No Hit
GATTTATGATATAACCAACAGACACTCCTGGACACCCACCCGGTTCTCAG32980.34929410394094407No Hit
CTCCTGGACACCCACCCGGTTCTCAGGCTCCCTCGTTGGGGGCAAAGCTG31950.33838528262320083No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAACGAG28070.29729185863014856No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT27620.2925258687340472No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCGAGCACCATTA27030.2862771264258253No Hit
TCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTA26110.2765333248604624No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCG24420.25863438502843705No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG23850.2525974644933753No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23780.25185608828731504No Hit
GTCCAGGAGTGTCTGTTGGTTATATCATAAATCAGTGTCCTGGGGGCTTG22600.23935860367087133No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21490.22760249526048784No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21120.22368379245702666No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA20070.21256314936612336No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19480.20631440705790147No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTTTAATGGTGCTC19210.20345481312024064No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18860.19974793208993952No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18780.19890064499729926No Hit
GACTATTACTGCTTGCTCTCCTTTAATGGTGCTCGGGTGTTCGGCGGGGG17850.18905093254535635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.1886272889990362No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16850.17845984388735317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16390.17358794310467174No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16110.17062243828043086No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15780.1671273790232898No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15470.16384414153930882No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15460.1637382306527288No Hit
ACACTGGACCTGTCACCAGCGGTCATTTCCCCTACTGGTTCCAGCAGAAG15370.1627850326735085No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15320.16225547824060835No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA15020.1590781516432074No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14620.15484171618000614No Hit
CTATTACTGCTTGCTCTCCTTTAATGGTGCTCGGGTGTTCGGCGGGGGGA14620.15484171618000614No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14560.15420625086052595No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCGAGCACCATTAA14520.15378260731420584No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14310.15155847869602518No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC14090.14922843919126447No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13920.14742795411940393No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13880.1470043105730838No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13220.14001419205880172No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13020.1378959743272011No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13020.1378959743272011No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12950.13715459812114086No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12630.13376544975057986No Hit
CAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGC11210.11872610385621539No Hit
GTCTGTTGGTTATATCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTC11000.11650197523803472No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10790.11427784661985406No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC10750.11385420307353394No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10740.1137482921869539No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10630.11258327243457356No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC10620.11247736154799351No Hit
CCAACAGACACTCCTGGACACCCACCCGGTTCTCAGGCTCCCTCGTTGGG10590.11215962888825343No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10420.11035914381639289No Hit
GTACATGGGGGCACCAGGGGTCCCTTCCAATATCAGCACCATGGCCTGGA10420.11035914381639289No Hit
GTATCAACGCAGAGTACATGGGGGCACCAGGGGTCCCTTCCAATATCAGC9890.1047458668276512No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9830.10411040150817104No Hit
TTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG9790.1036867579618509No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG9760.10336902530211081No Hit
CCTGGACACCCACCCGGTTCTCAGGCTCCCTCGTTGGGGGCAAAGCTGCC9660.10230991643631049No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9620.10188627288999037No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9530.10093307491077007No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)