FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944190
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAATGGTGCTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTA36490.38646882513053515No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC34280.3630625191963482No Hit
CTCCTGGACACCCACCCGGTTCTCAGGCTCCCTCGTTGGGGGCAAAGCTG32370.34283353985956216No Hit
CCATTAAAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGC32080.33976212414874124No Hit
GATTTATGATATAACCAACAGACACTCCTGGACACCCACCCGGTTCTCAG31720.3359493322318601No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT28570.30258740295915015No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAACGAG27320.28934854213664624No Hit
TCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTA24400.258422563255277No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCGAGCACCATTA24370.2581048305955369No Hit
GTCCAGGAGTGTCTGTTGGTTATATCATAAATCAGTGTCCTGGGGGCTTG23180.24550143509251315No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCG22740.24084135608299176No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22650.23988815810377148No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG21880.23173301983710906No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21320.22580201018862728No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20000.21182177316006312No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA19990.21171586227348307No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTTTAATGGTGCTC19280.20419618932630085No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18870.19985384297651954No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17650.1869327148137557No Hit
GACTATTACTGCTTGCTCTCCTTTAATGGTGCTCGGGTGTTCGGCGGGGG17470.18502631885531515No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17270.1829081011237145No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16270.17231701246571135No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15390.1629968544466686No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15250.16151410203454813No Hit
ACACTGGACCTGTCACCAGCGGTCATTTCCCCTACTGGTTCCAGCAGAAG15190.16087863671506794No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14560.15420625086052595No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA14190.1502875480570648No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14180.15018163717048474No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14180.15018163717048474No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14120.14954617185100458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14070.14901661741810443No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13770.14583929082070346No Hit
CTATTACTGCTTGCTCTCCTTTAATGGTGCTCGGGTGTTCGGCGGGGGGA13730.14541564727438333No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13600.14403880574884292No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13510.14308560776962262No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC13430.1422383206769824No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13380.14170876624408224No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCGAGCACCATTAA12950.13715459812114086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12410.13143541024581917No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12380.1311176775860791No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12300.1302703904934388No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12020.12730488566919795No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC11940.12645759857655767No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11410.12084432158781602No Hit
CAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGC10870.1151251337124943No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10680.1131128268674737No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC10670.11300691598089367No Hit
CCAACAGACACTCCTGGACACCCACCCGGTTCTCAGGCTCCCTCGTTGGG10460.110782787362713No Hit
GTCTGTTGGTTATATCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTC10460.110782787362713No Hit
GTTCTCAGGCTCCCTCGTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG10240.10845274785795234No Hit
CCTGGACACCCACCCGGTTCTCAGGCTCCCTCGTTGGGGGCAAAGCTGCC10130.10728772810557198No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10010.10601679746661159No Hit
GTACATGGGGGCACCAGGGGTCCCTTCCAATATCAGCACCATGGCCTGGA9900.10485177771423124No Hit
GTATCAACGCAGAGTACATGGGGGCACCAGGGGTCCCTTCCAATATCAGC9760.10336902530211081No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9670.10241582732289052No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9630.10199218377657038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.062.8374181
GTATCAA13450.059.541111
ATCAACG14350.053.7814833
TCAACGC14600.052.366544
TATCAAC15600.049.9344252
CAACGCA16250.047.0593265
CGCAGAG17250.045.1005948
ACGCAGA17600.044.2037127
AACGCAG17450.044.170876
ACACGAC800.002088699336.063753
GCAGAGT22400.034.090129
TATTGAG16750.030.1492425
TTATTGA17950.028.5295434
GTACATG24400.027.202841
TACATGG23650.026.2281822
ACATGGG23800.026.062883
GTACACG1959.0958165E-625.8974255
ATTGAGG9200.025.0558876
TTGAGGG9550.024.8919097
GTATTAC2651.4427133E-724.502641
Produced by FastQC (version 0.11.5)