FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065388
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTATCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA45260.42482175507890085No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39250.36841038194535697No Hit
GTACTGGGACACATGTCTCGCACAGTAATAAACAGCCGTATCTGCGGCGG35730.3353707757173912No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTGGTTACTACTGGGGCTGGATCCG34950.32804949933733063No Hit
ATCTATTATAGTGGGAGCACCTACCACAACCCGTCCCTCAAGAGTCGAAT33750.3167859972141605No Hit
CTATTATAGTGGGAGCACCTACCACAACCCGTCCCTCAAGAGTCGAATCA33720.31650440966108123No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT29420.27614352705305484No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28950.27173198872147986No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27390.2570894359613587No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT26690.2505190597228428No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG23430.21991987895489717No Hit
GTTTATTACTGTGCGAGACATGTGTCCCAGTACCGTGGGGCAGTACCAGC23400.21963829140181795No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22360.20987658956173713No Hit
ATTATAGTGGGAGCACCTACCACAACCCGTCCCTCAAGAGTCGAATCACC20520.1926058863062096No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG20380.1912918110585064No Hit
TCCCAGTACCGTGGGGCAGTACCAGCTGCCCGAGGTAGCCTCTACTACTA19890.18669254769154525No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19260.18077920907688091No Hit
CTATAATAGATACTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCG18210.17092364471910704No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17900.1680139066706214No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17180.16125580539671933No Hit
ATAATAGATACTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGA16980.15937855504285764No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16570.15553019181744115No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT16260.15262045376895553No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC15770.14802119040199438No Hit
CCGTAGTAGTAGTAGAGGCTACCTCGGGCAGCTGGTACTGCCCCACGGTA15620.1466132526365981No Hit
GAGTTTGTAGAGCTTGCATGCAGGAATAAATCCCAACATCCTCAGCCTCC15540.14586235249505344No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15390.14445441472965717No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15250.14314033948195398No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14900.13985515136269602No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14890.13976128884500294No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14080.13215842491186308No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13970.13112593721723917No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTGGTTACTACTGGG13830.12981186196953598No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT13150.12342921076640623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12900.12108264782407911No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12220.11469999662094936No Hit
GGATTGGGAGTATCTATTATAGTGGGAGCACCTACCACAACCCGTCCCTC11920.11188412109015683No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC11610.1089743830416712No Hit
CTCCATGTGTGTCCCCGATCAAGACATAGCCATCCGGGTCTTCGCCATCC11350.10653395758165102No Hit
TAGTAGTAGAGGCTACCTCGGGCAGCTGGTACTGCCCCACGGTACTGGGA11350.10653395758165102No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA11210.10521988233394783No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT10960.10287331939162071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10960.10287331939162071No Hit
GGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGACTGGAGTG10830.1016531066616106No Hit
CTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCG10710.1005267564492936No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10690.10033903141390742No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10670.10015130637852125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13350.066.455781
GGTATCA5250.063.1985971
TCAACGC14600.059.752684
ATCAACG14750.059.633833
TATCAAC15150.057.5834472
CAACGCA15700.055.5714235
AACGCAG16550.052.236
ACGCAGA18100.046.961317
CGCAGAG18450.046.070458
GTCATAG16600.040.8440361
GCAGAGT21350.040.8248189
TAGGTGG16600.039.5274775
ATAGGTG16650.038.1064
CATAGGT17050.037.6348843
TCATAGG17300.037.0910222
GGTGGTC17950.036.117267
GTACATG27350.035.3391721
GTGGTCA18550.035.3373768
TACACCG1251.31339E-534.6104355
TACATGG27500.034.6071742
Produced by FastQC (version 0.11.5)