FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587730
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG22390.3809572422711109No Hit
GATTATTACTGTGCAACATGGGATGACAGTGTGAATGGGGTGTTCGGCGG17440.29673489527504127No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12880.21914824834532864No Hit
ATATAGGGCCGTGTCCTCAGATGTCAGGCTGGTCAGCTCCATGTAGACTG11690.1989008558351624No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10310.1754206863695915No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTCACACTG10060.1711670324809011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.16657308628111547No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9730.16555220934782977No Hit
GCCCTATATTATTGTACGAGAAATCGTCTTTTAGATGATGACTTCGACCT9370.15942694774811564No Hit
GTCTACATGGAGCTGACCAGCCTGACATCTGAGGACACGGCCCTATATTA8770.1492181784152587No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC7820.13305429363823523No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7740.1316931243938543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7410.126078301260783No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG7320.12454698586085447No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7230.12301567046092594No Hit
GATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG7200.12250523199428308No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7020.11944260119442603No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6990.11893216272778316No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA6920.11774113963894986No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6840.11637997039456893No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6720.11433821652799755No Hit
ATATTATTGTACGAGAAATCGTCTTTTAGATGATGACTTCGACCTGGGAC6610.11246660881697379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.069.586081
TCAACGC13950.057.2954034
ATCAACG14400.055.5096443
CAACGCA14300.055.384815
TATCAAC14650.055.0539322
AACGCAG15600.050.7694136
CGCAGAG16950.046.717888
GGTATCA5950.043.5928541
GCAGAGT18950.041.787239
ACGCAGA19450.040.713017
GTACATG15550.027.8003731
TTAGTAC1358.403411E-426.67133
CTTAGTC1100.00999159326.1863672
GTATAGG1701.07518965E-425.4291671
CAGAGTA18600.025.1570999
TATTGAG7550.024.794795
CCCTATA6500.024.3734652
TATATAG2401.6927315E-624.0041712
ACGCCGT3352.2810127E-923.6418746
GGTTTAT6450.023.4579141
Produced by FastQC (version 0.11.5)