FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587730
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGTGTGAATGGGGTGTTCGGCGG17960.3055824953635172No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG13880.23616286390009017No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13090.2227213176118286No Hit
ATATAGGGCCGTGTCCTCAGATGTCAGGCTGGTCAGCTCCATGTAGACTG12140.20655743283480513No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC11480.19532778656866245No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10530.17916390179163902No Hit
GCCCTATATTATTGTACGAGAAATCGTCTTTTAGATGATGACTTCGACCT9680.1647014785700917No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTCACACTG8940.15211066305956816No Hit
GTCTACATGGAGCTGACCAGCCTGACATCTGAGGACACGGCCCTATATTA8690.14785700917087777No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8340.14190189372671125No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG8240.1402004321712351No Hit
GATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG7360.12522757048304495No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC7270.1236962550831164No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC7170.12199479352764024No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7090.12063362428325931No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6760.115018801150188No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA6640.11297704728361663No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC6610.11246660881697379No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6400.10889353955047386No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6280.10685178568390248No Hit
ATATTATTGTACGAGAAATCGTCTTTTAGATGATGACTTCGACCTGGGAC6240.106171201061712No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6090.10361900872849779No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT6080.10344886257295018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7450.055.1147421
ATCAACG8300.045.1232643
TCAACGC8350.044.845434
TATCAAC8950.042.658152
CAACGCA8850.042.3117875
AACGCAG9150.040.9140666
GGTATCA5050.034.23491
CGCAGAG11100.033.726468
GTATGTA5400.033.349971
TATAGGG7250.032.788772
TAGGGCC7250.031.7843464
GTACACG3650.031.5666455
ATATAGG7450.030.941611
TACACGG3900.029.5355976
TAGAACC1753.9749793E-628.8045624
ACGGGGT4300.028.4623589
CCGTGTC7950.028.072759
ACGCAGA13350.028.0422257
ATAGGGC8650.027.4772363
GCAGAGT14300.027.1861769
Produced by FastQC (version 0.11.5)