FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125073
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCGACTCCCAGGCTGTTCATTTGCAGAAACA23980.21314172502584278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.14505725406262526No Hit
GTCTATTACTGTGCGAAGTATGGCCTAGCCTATCCCAGCAGTCTGACAGG12770.1135037459791498No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11310.10052681026031199No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24400.060.8509864
CAACGCA25300.058.9712145
ATCAACG25800.057.5489963
GTATCAA26700.056.9639471
AACGCAG26550.056.1922876
CGCAGAG31250.047.9204338
TATCAAC34000.043.881522
GCAGAGT38000.039.4082539
ACGCAGA39500.037.9285937
GGTATCA15500.035.8086471
TAGACGG12600.031.4617145
ATAGACG12950.030.6113974
ACCGACC2901.2732926E-1129.7913868
GACGGCC12850.029.7079877
GTACATG27700.028.364531
AGACGGC13750.028.3049246
TACATGG27700.027.8416042
GTTACGC1050.00797585227.4269899
CCGACCG3153.8198777E-1127.4269899
ACATGGG28800.025.2766273
Produced by FastQC (version 0.11.5)