FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125073
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCGACTCCCAGGCTGTTCATTTGCAGAAACA22410.19918707497202404No Hit
GTCTATTACTGTGCGAAGTATGGCCTAGCCTATCCCAGCAGTCTGACAGG12410.1103039536101213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13150.054.8023384
ATCAACG14250.050.5742343
CAACGCA15150.047.571945
AACGCAG15650.046.4815336
CGCAGAG18100.039.7919168
GTATCAA22600.033.497971
TAGCGCG1102.5325184E-432.7378968
GTACATG28000.029.8701651
GCAGAGT24900.029.2142989
TACATGG28450.028.8779772
TATCAAC26300.027.676412
ACGCAGA26300.027.3853117
ACATGGG28450.026.0915053
ATAGACG10800.026.0234994
TAGACGG10650.025.715655
GACGGCC12250.024.105787
GTATTAG7200.022.0306781
GAATACG2303.27942E-521.9435981
GGTATCA16400.021.542191
AGACGGC13400.021.4995126
Produced by FastQC (version 0.11.5)