FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945845
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGGCCGTGTCGTCAGCTCTCAGATTGTTCATTTGCAGCTGAAGGG43500.4599062214210574No Hit
ATATTATACTACTCCGTACACTTTTGGCCAGGGGACCAGACTGGAGATCA42930.4538798640369194No Hit
ATACTACTCCGTACACTTTTGGCCAGGGGACCAGACTGGAGATCAAACGA34590.3657047402058477No Hit
GCCTTATATTATTGTGTAAAAGAGATCCTATCTGGGGGCTTTGAGTACTG33510.35428637884642833No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCCGCAGGCT29140.308084305568037No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25780.2725605146720657No Hit
ATTTTATACACCTCCAAGAATAAGAACGAATTAGCTTGGTATCAGCAGAA24670.260824976608218No Hit
TCCATACAGTGATCAGCACTGAGCACAGAGGACTCACAATGGAGTTGGGA24230.2561730516099361No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCCGC20420.21589161014754005No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18460.19516939879155676No Hit
CAATAATATAAGGCCGTGTCGTCAGCTCTCAGATTGTTCATTTGCAGCTG16040.16958381130100597No Hit
TTATACTACTCCGTACACTTTTGGCCAGGGGACCAGACTGGAGATCAAAC15340.16218302153101194No Hit
ATATTATTGTGTAAAAGAGATCCTATCTGGGGGCTTTGAGTACTGGGGCC14130.14939022778573657No Hit
GATCAGCACTGAGCACAGAGGACTCACAATGGAGTTGGGACTGAGCTGGA14110.14917877664945103No Hit
CTTTTACACAATAATATAAGGCCGTGTCGTCAGCTCTCAGATTGTTCATT13610.14389249824231243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.13733751301746058No Hit
GTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCCGCAG12330.13035962552003763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12300.1300424488156093No Hit
GTGTAAAAGAGATCCTATCTGGGGGCTTTGAGTACTGGGGCCGGGGAACC12190.12887946756603882No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12160.12856229086161053No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12070.12761076074832556No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12060.1275050351801828No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTCCATGGCAAAATCAC11980.12665923063504061No Hit
TTATTACTGTCAGCAATATTATACTACTCCGTACACTTTTGGCCAGGGGA11870.12549624938547013No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11830.12507334711289902No Hit
CCTCCAAGAATAAGAACGAATTAGCTTGGTATCAGCAGAAACCAGGACAG11390.1204214221146171No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11190.11830691075176164No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10980.11608667382076346No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10820.11439506473047908No Hit
GCCTGGCAGGTCCCTCAGACTCTCCTGTGCAGCCTCTGGATTCACGTTTG10380.10974313973219714No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10290.1087916096189122No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10260.10847443291448387No Hit
GTAATAAACTGCCACATCTTCAGCCCGCAGGCTGCTGATGGTGAGAGTGA10250.1083687073463411No Hit
GTACATGGGAGGTCTGGGTGAGGAGCCCCAGCCCTGAGATTCCCAGGTGC9900.1046683124613441No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9680.10234234996220311No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)