FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945845
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGGCCGTGTCGTCAGCTCTCAGATTGTTCATTTGCAGCTGAAGGG42670.45113099926520733No Hit
ATATTATACTACTCCGTACACTTTTGGCCAGGGGACCAGACTGGAGATCA41810.442038600404929No Hit
GCCTTATATTATTGTGTAAAAGAGATCCTATCTGGGGGCTTTGAGTACTG33320.35227759305171563No Hit
ATACTACTCCGTACACTTTTGGCCAGGGGACCAGACTGGAGATCAAACGA31420.332189735104589No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCCGCAGGCT30160.3188683135185998No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24790.2620936834259313No Hit
ATTTTATACACCTCCAAGAATAAGAACGAATTAGCTTGGTATCAGCAGAA23660.25014669422579805No Hit
TCCATACAGTGATCAGCACTGAGCACAGAGGACTCACAATGGAGTTGGGA23030.24348598343280348No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCCGC21210.224243930030819No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18010.19041174822513204No Hit
CAATAATATAAGGCCGTGTCGTCAGCTCTCAGATTGTTCATTTGCAGCTG15260.16133721698586978No Hit
TTATACTACTCCGTACACTTTTGGCCAGGGGACCAGACTGGAGATCAAAC15020.15879980335044325No Hit
ATATTATTGTGTAAAAGAGATCCTATCTGGGGGCTTTGAGTACTGGGGCC13730.1451612050600257No Hit
GATCAGCACTGAGCACAGAGGACTCACAATGGAGTTGGGACTGAGCTGGA13500.14272951699274194No Hit
GTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCCGCAG12880.1361745317678901No Hit
CTTTTACACAATAATATAAGGCCGTGTCGTCAGCTCTCAGATTGTTCATT12690.1341657459731774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12160.12856229086161053No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11990.1267649562031834No Hit
GTGTAAAAGAGATCCTATCTGGGGGCTTTGAGTACTGGGGCCGGGGAACC11670.12338173802261469No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11600.12264165904561529No Hit
TTATTACTGTCAGCAATATTATACTACTCCGTACACTTTTGGCCAGGGGA11430.12084432438718817No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11170.11809545961547611No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11170.11809545961547611No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTCCATGGCAAAATCAC11000.116298124957049No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10940.11566377154819235No Hit
CCTCCAAGAATAAGAACGAATTAGCTTGGTATCAGCAGAAACCAGGACAG10850.11471224143490741No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10810.11428933916233631No Hit
GTAATAAACTGCCACATCTTCAGCCCGCAGGCTGCTGATGGTGAGAGTGA10250.1083687073463411No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10010.10583129371091457No Hit
GTACATGGGAGGTCTGGGTGAGGAGCCCCAGCCCTGAGATTCCCAGGTGC9760.10318815450734528No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9650.1020251732577748No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9530.10075646644006153No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9520.10065074087191876No Hit
GCCTGGCAGGTCCCTCAGACTCTCCTGTGCAGCCTCTGGATTCACGTTTG9500.10043928973563322No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)