FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668699
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA37920.22724290000773056No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35470.21256080335638725No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT32660.195721337401173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.17774325986891584No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG27970.1676156095257443No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC24830.1487985550419818No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22690.13597419306897168No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT21840.13088040443483218No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG21820.13076055058461714No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG19720.1181758963120371No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19070.11428064618004806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18710.1121232768761772No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18420.110385396048059No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18240.10930671139612357No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17350.10397321506155394No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16770.10049745340531757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.074.719261
GGTATCA14600.070.524631
TCAACGC40750.060.2574044
ATCAACG41200.059.6010443
TATCAAC42400.057.2348672
CAACGCA43200.056.5066575
AACGCAG46050.053.00956
CGCAGAG51800.046.7082068
ACGCAGA56550.042.9122167
GCAGAGT68000.035.8886649
TATTGAG20600.031.1104625
GTACATG46450.028.3676261
ATTGAGT15450.027.964466
ACATGGG45700.027.8903353
TTATTGA23100.027.7435284
TACATGG47050.026.783982
GAATACG2207.9679194E-726.1778419
GTAATAC24050.026.0472451
CAGAGTA57750.025.8038739
AATACAC25950.024.4197943
Produced by FastQC (version 0.11.5)