FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913923
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.24630083716024215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.11729653373424237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.1131386342175435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25950.081.8961941
GGTATCA11600.065.830471
TCAACGC34000.061.87384
ATCAACG34400.061.363773
CAACGCA36650.057.5965585
AACGCAG38600.054.6838956
TATCAAC40150.052.5756842
CGCAGAG40000.052.5610778
ACGCAGA43800.048.1838267
GCAGAGT43850.047.943639
GTACATG38500.034.429931
TACATGG39250.032.489012
ACATGGG39700.031.9392743
CAGAGTA44550.027.7970319
CATGGGG22900.027.3707454
TATACCG1856.109789E-627.2587176
TACGCTA3701.0913936E-1125.296439
AATGCGG1751.3124074E-424.695687
CATGGGT10850.024.5682854
GTACACG5250.023.3288025
Produced by FastQC (version 0.11.5)