FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121310
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGA50290.23707048946170053No Hit
ACCATATACTACGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGA48130.22688810216328592No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTATTCATTTGCAAATACA39060.18413150364633174No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35850.16899934474452108No Hit
GTATATGGTACTACTAGTACGACTAATGTATGAAACCCACTCCAGCCCCT35660.16810367178771607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33900.15980691176678563No Hit
GTGTATTACTGTGCGAGAGATGACTACACTGACTACTGGGAGGACCGTTA31520.14858742946575465No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGCCT29210.13769793193828342No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25080.11822883029825909No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGCC22720.10710362936110233No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC22080.10408662571712764No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21560.10163531025639816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49600.065.6453551
TCAACGC51750.061.9159244
ATCAACG52100.061.638183
CAACGCA55250.057.9936455
GGTATCA26250.054.609851
AACGCAG59100.054.213166
TATCAAC63800.050.8989142
CGCAGAG66600.048.215058
GCAGAGT75450.042.464189
ACGCAGA98400.032.6333547
GTACATG65250.030.6910341
CGTTATA950.00489183930.3150567
TACATGG66400.029.1700332
ACATGGG65650.028.845213
CAGAGTA77550.025.4384569
CAGCCGT28400.024.5909569
ACTACGC30900.023.3004068
CATGGGG42350.023.29274
GTATATA10100.022.8231641
GTATAAT42700.022.6060241
Produced by FastQC (version 0.11.5)