FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329293
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG69180.29699999098438884No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC63160.2711552389501879No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC56010.24045922947435122No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG54750.23504986276951848No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG52160.22393060898736228No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50720.21774847561041055No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT49650.21315480705948112No Hit
GATTATTACTGCAGCTCATCTGCAGACAACAACAATGTGGTCTTCGGCGG49170.21109409593383058No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG46640.20023243104238067No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC43700.1876105753977709No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATCT42100.180741538312269No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT41340.1774787456966556No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATTGTTGTTG41150.17666304754275225No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA40660.1745594049353173No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40400.17344318640892323No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39800.17086729750186No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTCTAACTTTGTCTTCT39780.17078143453829123No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA38060.1633972196713767No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA35780.15360884182453646No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT35650.15305073256133944No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT35450.1521921029256517No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA34380.14759843437472228No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34210.14686859918438772No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34150.1466110102936814No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT33910.1455806547308561No Hit
GTCAATAAGCGGCCCTCGGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC33590.14420684731375571No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33400.14339114915985238No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG33400.14339114915985238No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC32320.13875454912713858No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT31960.13720901578290065No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC31640.13583520836580026No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG31640.13583520836580026No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG31600.13566348243866272No Hit
ATATAAGGCCGTGTCGTCGGCCCTCAGGCTGTTCATTTGGATATACATTG31400.134804852802975No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG30380.13042584166096752No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGGTT29790.12789288423568868No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29530.12677666570929463No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29470.1265190768185883No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT29070.12480181754721281No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC28560.12261231197620909No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA28280.12141023048624626No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG27800.1193495193605957No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT27280.11711708230780755No Hit
GCCTTATATTTCTGTGCGAGGAGAGTAAATTGGGCTTTTGACTACTGGGG27210.11681656193531685No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTCTA26670.11449826191895997No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA25970.11149305819405289No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC24630.10574023963494503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.10432350073606025No Hit
TTGTTGTTGTCTGCAGATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC24030.10316435072788181No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG23750.10196226923791897No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG23720.10183347479256581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.083.498151
GTATCAA40300.067.178071
ATCAACG42400.061.4707873
TCAACGC43250.060.262694
TATCAAC44900.058.5292052
CAACGCA48050.054.392545
AACGCAG50400.052.1432046
ACGCAGA52450.049.8306477
CGCAGAG54450.048.397028
GCAGAGT59350.044.39949
GAATACG7550.037.190719
GTCATAG32950.036.2741621
GTACATG53500.035.5299721
ATAGGTG32600.035.1160474
TACATGG54050.034.90052
ACATGGG54100.034.4689943
TAGGTGG33800.034.2953535
TATTGAG36950.033.5150685
CAGAGCG31300.033.3534249
GCACACA31800.031.9240572
Produced by FastQC (version 0.11.5)