FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977441
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTATGCTGACTCCGTGGAGGGCCGATTCACCATTTCCAGAGA67520.34145140107846456No Hit
ATACTATGCTGACTCCGTGGAGGGCCGATTCACCATTTCCAGAGACAATG67030.33897345104101717No Hit
GTATATATGATTTTCATCATGTGATATAACTGCCACCCATGCCAGCCCCT62900.3180878721539606No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT52450.2652417948247255No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA51210.25897106411771575No Hit
TTTAAGAGGTGTCCAATGTCAGAAACAGGTGGTGGAGTCGGGGGGAGGCC50890.25735281103203583No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC48210.24379994143946646No Hit
GTCTATTTCTGTGTGAAAGGAAGGGGACTAGTACCACATCCGTTGCCGGG45510.2301459310290421No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC45060.2278702626273047No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44530.2251900309541473No Hit
GCATAGTATATATGATTTTCATCATGTGATATAACTGCCACCCATGCCAG40990.20728810619381313No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG38810.1962637570476186No Hit
GAAATAGACAGCCGTGTCCTCAATTTTCAGGCTGTTCATTTCCAAATAAA38620.19530291927799615No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38170.19302725087625877No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC37790.19110557533701383No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT36150.18281202827290421No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC36010.18210404254791926No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33470.16925915868033484No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG30110.1522675012806956No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT30020.1518123676003481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.15161008596463815No Hit
CTCCTGTGCAGTCTCTGGATTCGCCTTCAGTAATTTTGCCATGAACTGGG28800.1456427777111934No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA27890.1410408704987911No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27300.13805721637206875No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT26050.13173591525613154No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC25730.1301176621704516No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG25580.12935910603653913No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT25400.12844883867584417No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA25330.1280948458133517No Hit
GTCTCTGGATTCGCCTTCAGTAATTTTGCCATGAACTGGGTCCGCCAGGC24330.12303780492060193No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23930.12101498856350201No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG23800.12035757324744456No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23640.11954844670460459No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG23510.11889103138854713No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC23320.11793019361892465No Hit
GTACTAGTCCCCTTCCTTTCACACAGAAATAGACAGCCGTGTCCTCAATT23170.1171716374850122No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC23130.11696935584930221No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC22970.11616022930646223No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA22870.11565452521718728No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG22740.1149971099011298No Hit
CGTCCATACCGTAGAAGTAATAGTCAGCCGGACCCGGCAACGGATGTGGT22380.11317657517973988No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC22380.11317657517973988No Hit
GACTAGTACCACATCCGTTGCCGGGTCCGGCTGACTATTACTTCTACGGT22050.11150775168513244No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT21800.11024349146194501No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT21760.11004120982623501No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC21400.1082206751048451No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACCAGAAGTCGGCGGTGTT21280.10761383019771513No Hit
TCCAGAGACAATGCCAAGAACATACTTTATTTGGAAATGAACAGCCTGAA21130.10685527406380266No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC20890.10564158424954272No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT20630.10432675361742778No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCGCCTTCAGTAATTTTGCC20450.1034164862567328No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT20390.10311306380316784No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG20150.10189937398890787No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT20100.1016465219442704No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)