FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977441
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATGCTGACTCCGTGGAGGGCCGATTCACCATTTCCAGAGACAATG63000.3185935762432356No Hit
CATATATACTATGCTGACTCCGTGGAGGGCCGATTCACCATTTCCAGAGA57930.2929543789169942No Hit
GTATATATGATTTTCATCATGTGATATAACTGCCACCCATGCCAGCCCCT57650.2915384074670243No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT51650.2611961621105257No Hit
TTTAAGAGGTGTCCAATGTCAGAAACAGGTGGTGGAGTCGGGGGGAGGCC48430.24491249043587143No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41980.2122945766776354No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA40430.20445616329387326No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40080.20268619898141083No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC40050.20253448775462832No Hit
GTCTATTTCTGTGTGAAAGGAAGGGGACTAGTACCACATCCGTTGCCGGG40040.20248391734570084No Hit
GCATAGTATATATGATTTTCATCATGTGATATAACTGCCACCCATGCCAG36330.18372229563359918No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA36070.18240746500148425No Hit
GAAATAGACAGCCGTGTCCTCAATTTTCAGGCTGTTCATTTCCAAATAAA35630.18018236700867435No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC35150.17775498738015447No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC30350.15348119109495553No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30160.1525203533253331No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29300.1481712981575683No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT29230.1478173052950758No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT28510.14417623585229597No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28080.14200170826841357No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA27670.13992832150238615No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27070.1368940969667363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26160.13229218975433402No Hit
CTCCTGTGCAGTCTCTGGATTCGCCTTCAGTAATTTTGCCATGAACTGGG25870.13082564789543658No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT24990.12637545190981678No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24280.12278495287596444No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG23990.121318411017067No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22950.11605908848860724No Hit
GTACTAGTCCCCTTCCTTTCACACAGAAATAGACAGCCGTGTCCTCAATT22500.11378342008686985No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT22280.1126708710904649No Hit
GTCTCTGGATTCGCCTTCAGTAATTTTGCCATGAACTGGGTCCGCCAGGC21860.11054691391551No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC21850.1104963435065825No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA21750.10999063941730751No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC21700.10973778737267002No Hit
CGTCCATACCGTAGAAGTAATAGTCAGCCGGACCCGGCAACGGATGTGGT21440.10842295674055509No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG21260.10751268937986014No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG20670.10452903525313777No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC20000.10114081785499542No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACCAGAAGTCGGCGGTGTT19880.10053397294786544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.086.5184561
GTATCAA38250.074.731421
TCAACGC40700.068.999724
ATCAACG40950.068.754323
TATCAAC43000.065.4764942
CAACGCA46400.060.368275
AACGCAG48150.058.4732936
CGCAGAG49600.056.7638978
GCAGAGT58950.047.507929
ACGCAGA64950.043.4594277
GAATACG4500.043.1967939
CAGCGCT13100.034.6233839
GTACATG44900.032.7135731
TACATGG45150.031.2590272
TATAGCG2559.640644E-1131.0620164
ATATCCG1204.224877E-430.0030845
ACATGGG48000.028.8029613
CATGGGA25050.027.8831044
GGCAGCG16800.027.4313897
GTGCTAG8400.027.4293061
Produced by FastQC (version 0.11.5)