FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029939
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCACACGA72190.3556264498588381No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT62390.3073491370922969No Hit
GGGTAGAATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC48610.23946532383485414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43960.2165582315527708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.15872398136101626No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT31530.1553248644417394No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30710.1512853341898451No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27630.13611246446321787No Hit
GTGTATATGAATCTGGGCAGCCTGACATCGGAGGACACGGCCGTGTATTA27230.13414196190131822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27050.13325523574846337No Hit
GTAATACACGGCCGTGTCCTCCGATGTCAGGCTGCCCAGATTCATATACA26780.1319251465191811No Hit
GTATATGAATCTGGGCAGCCTGACATCGGAGGACACGGCCGTGTATTACT26040.1282797167796668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25620.12621068908967215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25160.12394461114348757No Hit
GACTTATTCTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA24780.12207263370968291No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24590.12113664499278057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24450.12044696909611569No Hit
ACCAGGGACAGCCCCTAGGCTCCTGATCCATTCTGCATCCACTTTGCACA24190.11916614243088093No Hit
CATATACACTGTGGTCACTGTCGTCGTGGATTCGTCCGCGGTAATCGTGA24050.11847646653421605No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24030.11837794140612107No Hit
TTCATATACACTGTGGTCACTGTCGTCGTGGATTCGTCCGCGGTAATCGT23290.11473251166660672No Hit
GTGTATTACTGTGCGAGAGGCTTCGAGGGCCTATTCGGGAGGGCCTCCGA21810.10744165218757806No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21350.10517557424139345No Hit
AACCAGGGACAGCCCCTAGGCTCCTGATCCATTCTGCATCCACTTTGCAC21270.10478147372901354No Hit
ACTTTGCACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGAC21220.10453516090877608No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC21070.10379622244806372No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20540.10118530655354668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41450.074.863081
TCAACGC49450.062.0298964
ATCAACG50350.061.2071383
TATCAAC52250.059.2570342
CAACGCA53500.057.4687655
AACGCAG55250.055.6484836
CGCAGAG61000.049.8127338
ACGCAGA65350.046.6071477
GCAGAGT70150.043.3100859
GGTATCA40100.034.4724241
GTACATG53300.034.310061
TACATGG53700.032.9851572
ACATGGG55350.031.871773
GAATACG5400.030.6646739
CATGGGA28000.027.5158864
CATTAGC16300.026.9463528
TCTACAC14250.026.2752113
GTGCTAG9200.025.8250431
CATGGGG33050.025.2722724
ATACACG16950.025.0634064
Produced by FastQC (version 0.11.5)