FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371687
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36450.15368807098069856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49450.073.819091
TCAACGC56700.063.2386324
ATCAACG56950.062.9610253
GGTATCA21750.059.2544251
TATCAAC63550.056.7620852
CAACGCA70200.051.1799245
ACGCAGA70600.050.4820147
AACGCAG72350.049.559516
CGCAGAG75250.047.458218
GCAGAGT90150.039.450429
GTACATG67700.029.990761
TACATGG71150.027.8287682
ACATGGG69900.027.1933633
CAGAGTA85900.026.3164279
TAGACCG1450.001277379124.8277975
GCCCATA13400.024.7161061
CATGGGG49450.022.8596484
TAGTACG4007.530616E-1021.6001834
AATCCGC11400.019.5791136
CATGGGA33250.019.4888884
Produced by FastQC (version 0.11.5)