FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849831
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTACACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGACATCA23740.27934965893218766No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22380.2633464771230986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19310.2272216475981695No Hit
GTAGTACTATAGCTTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCC17360.20427590897484327No Hit
GTGTAATCATGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGGCT16690.1963919885247773No Hit
GATTTACACTGGTATCAACTGAAGCCCGGGAGAGCCCCTAATCTCCTGAT14570.17144585217531486No Hit
GGTATCAACTGAAGCCCGGGAGAGCCCCTAATCTCCTGATCTATCGTGCA14250.16768039763199977No Hit
GTATTATTGTGCAAAGGTACTTCTGTTCCACGGTGCTTTTGATACCTGGG13380.1574430680923619No Hit
TAGTACTACTGTCCCAACTAATTCCTGACACCCACTCCAGGCCCTTCCCT12800.1506181817326033No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12640.14873545446094574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.13696840901308613No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11600.13649772719517175No Hit
CAATAATACAGGCCCGTGTCCTCAGCGCGCAGGCTGTTCATGTGCAAAAA11540.13579170446830016No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10620.1249660226562693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10570.12437767038387632No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10510.12367164765700474No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10500.12355397720252616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10450.12296562493013317No Hit
GTTGGGGACAGAGTCACCATCTCTTGCCGGACAAGTCAGGACATTAGAGA10380.12214193174878299No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10230.12037687493160404No Hit
ATACAGGCCCGTGTCCTCAGCGCGCAGGCTGTTCATGTGCAAAAAGAGGG10090.11872948856890371No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT10050.1182588067509893No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9860.116023068115896No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9100.10708011357552266No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAACCTGAAGATTTTGCAACTTA9090.10696244312104408No Hit
ATAGTACTACTGTCCCAACTAATTCCTGACACCCACTCCAGGCCCTTCCC8960.1054327272128223No Hit
ACTTATTACTGTCAACATGATTACACTTACCCGTGGGCGTTCGGCCAAGG8720.102608636305336No Hit
CACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGACATCAACCGAA8720.102608636305336No Hit
GTGTAAATCATCTCTAATGTCCTGACTTGTCCGGCAAGAGATGGTGACTC8720.102608636305336No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT8590.10107892039711425No Hit
GTACTATAGCTTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGA8560.10072590903367847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19100.049.789823
TCAACGC19100.049.789824
CAACGCA20200.047.7918055
AACGCAG20850.045.953656
CGCAGAG23050.041.543168
ACGCAGA23100.041.467887
GCAGAGT28550.033.7922829
TACATGG22050.032.673182
GTACATG22350.032.2384071
ACATGGG22450.031.12833
CAGAGTA25800.029.8595249
TATCAAC36750.026.0731982
GTATCAA37950.025.251721
GTAGTAC12900.024.5761641
GGTATCA31600.024.3976421
CTAGTAC1500.001549954824.0147883
GTACTAT13450.023.5684174
AAGCGCT2151.9683786E-523.4411249
TACTATA13800.022.9706675
ACTATAG13350.022.6643096
Produced by FastQC (version 0.11.5)