FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775845
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT38970.21944482767358636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37790.2128001036126464No Hit
GTAATAAATGGCCGTGTCGTCGACTCTCAGGCTGTCCATTTGCAGAAACA36270.204240798042622No Hit
AATACATACTATGCAGCGTCTGTGCAGGGGCGCTTCTCCATGTCCAGAGA35650.2007495023495857No Hit
ATTTATTACTGCGCACGAACCCACTGGAACGTCGGAGAGGGTTGGTTCGG35370.19917278816563383No Hit
GTATGTATTGGAGCCACTGTCACTAATGCCTGAGACCCACTCCAGCCCCT34570.19466789049719993No Hit
ATACTATGCAGCGTCTGTGCAGGGGCGCTTCTCCATGTCCAGAGACAATT32270.18171630970045247No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31830.17923861598281382No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30630.17248126948016296No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30540.17197446849246414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29770.1676385044865965No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28460.16026173455453602No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28240.15902288769571668No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24750.1393702716171738No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23810.13407701685676396No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22820.128502205992077No Hit
GCATAGTATGTATTGGAGCCACTGTCACTAATGCCTGAGACCCACTCCAG22490.12664393570384802No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22330.12574295617016126No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22150.12472935419476364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22040.12410993076535397No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA22020.12399730832364311No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21650.12191379315199243No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT21590.12157592582685989No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21220.1194924106552092No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20280.11419915589479937No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20260.11408653345308853No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20060.11296030903598005No Hit
GCCTGAGAGTCGACGACACGGCCATTTATTACTGCGCACGAACCCACTGG19860.1118340846188716No Hit
GTTTTCACTCTCACCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTA18340.10327477904884716No Hit
GGACTGAACAGAGAGAATCCACCATGGAGTTTGGCCTGACGTGGCTTTTT18320.1031621566071363No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17960.10113495265634107No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)