FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820602
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC68310.3752055638739274No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG47120.25881549070032883No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC44130.24239235154086394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42770.2349222949332144No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT41050.22547487040001057No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG40590.22294823360624674No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35730.19625376661126376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.18905834443771896No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA32760.17994048122544082No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32640.17928135858358937No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT31580.1734591085805684No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA31200.1713718868813722No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA30010.16483558734967885No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT30010.16483558734967885No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC28990.1592330448939417No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT28460.1563219198924312No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27760.1524770378149645No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26140.14357888214997017No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTG25620.14072268403528063No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25430.13967907318568254No Hit
GTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA25310.13901995054383112No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25240.13863546233608443No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA24800.13621867931596252No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA24690.13561448356093203No Hit
GCTCATATACAAGCACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTG23740.13039642931294154No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23100.12688110855640056No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG22710.12473895997038341No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG22560.12391505666806914No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTGC22370.12287144581847104No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTC22270.12232217695026151No Hit
TCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCT22190.12188276185569388No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22050.12111378544020054No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG21640.1188617830805415No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20790.11419299770076051No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT20780.11413807081393956No Hit
TATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG20570.11298460619069957No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20530.11276489864341574No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT20030.11001855430236812No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA19780.1086453821318443No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCT19490.10705250241403667No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC18300.10051620288234332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.077.8463751
GTATCAA38050.075.1537861
TCAACGC44850.062.614374
ATCAACG45450.061.787783
TATCAAC47900.058.776152
CAACGCA47950.058.5646975
AACGCAG49750.056.5905086
ACGCAGA57200.049.0913167
CGCAGAG57650.048.708128
GCAGAGT78650.035.885869
TATTGAG29850.030.3938775
GTACATG48600.027.715351
CAGAGTA64300.027.0981989
TTATTGA34350.026.62254
ACATGGG50050.026.6157973
TACATGG49500.026.3289342
GAATACG4150.026.024319
CATGGGG33950.023.3305364
ATTGAGT22900.022.3245536
TTGAGGG17150.022.2509127
Produced by FastQC (version 0.11.5)