FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922277
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAAGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG17100.18541067379973694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.15711114990398764No Hit
TTCTATTACTGTGCGACCGTTTACTATTTAGCAGCAGCTCCTACCTACTA12790.13867850981863367No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11980.1298958989544356No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTATACTTGGGTGTTCGGCGG10090.10940314027130678No Hit
GTTATAAATATGTCTCCTGGTACCAACACCACCCAGGGAAAGCCCCCAAA9570.10376492095108085No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCC9290.10072895670172845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18950.068.066831
ATCAACG22600.056.1171953
TCAACGC22650.055.675174
CAACGCA23300.053.8127335
AACGCAG24450.051.2788776
TATCAAC25250.050.2276652
GGTATCA10100.049.9422841
CGCAGAG28050.044.9179578
ACGCAGA30250.041.431167
GTAATAG11150.036.1913571
AAGGCCG12050.032.2642488
GCAGAGT39100.032.223759
TATACCG1153.276931E-431.330255
TAATAGA12750.031.0845242
GTACATG26600.028.7154711
CTAGATC2054.2596184E-728.1208133
TATTGAG8750.028.0002965
GAACGTA1306.751826E-427.6911819
CAGAGTA32200.027.2783959
GTATAGG3453.6379788E-1227.1528851
Produced by FastQC (version 0.11.5)