FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804121
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGTCTGTGTGCACAGAAATATGTGGCTGTGTCCACAGGGTCCATG30780.38277821372654114No Hit
GACTTATACCACGTAGCCTCTTTGACTACTGGGGCCCGGGAACCCTGGTC23370.29062790301459607No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22390.27844068243460873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20920.26015985156462773No Hit
CTAATGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAGGGCCCTG16970.21103789106365836No Hit
CCACTAATGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAGGGCC14320.1780826517402232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14280.17758521416552983No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13310.16552235297921583No Hit
GGATATTAACAGGTGGTTAGCCTGGTATCAACAGAAGCCAGGGACAGCCC12810.1593043832955488No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12800.15918002390187547No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12640.15719027360310203No Hit
GTTAATATCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG11550.14363509969270793No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11490.1428889433306679No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11000.13679533304067423No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10960.13629789546598087No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10470.1302042851759872No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10390.1292094100266005No Hit
GAAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATCGTAAGAACCA10080.12535426882272693No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9780.12162348701252673No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9730.12100169004416002No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAACAG9500.1181414239896732No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9360.11640039247824643No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9320.11590295490355308No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8860.11018242279457942No Hit
GGTATCAACAGAAGCCAGGGACAGCCCCTAAACTCCTGATCTATGATGCA8790.10931190703886605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.10893882885784602No Hit
GGGTCCATGTTGGTCATCGTAAGAACCACTTGATTTTTGGAGGCGTCCTT8640.10744651613376593No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC8600.10694907855907257No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8600.10694907855907257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8490.10558112522866583No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8480.10545676583499249No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8380.10421317189825909No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8250.10259649978050568No Hit
ATTGTCAACAGGCTAACTTTTTCCCTCTCACTTTCGGCGGAGGGACCAAG8250.10259649978050568No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8240.10247214038683232No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8160.1014772652374456No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8130.1011041870564256No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8130.1011041870564256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18050.062.7002834
ATCAACG18200.062.1835173
CAACGCA18750.059.9750185
AACGCAG19650.057.2209436
CGCAGAG22650.049.9074138
GCAGAGT24550.045.455549
ACGCAGA31100.036.5970767
GTATCAA32850.035.3339041
GTACATG22600.035.0901641
GTGCTAG3700.035.0729181
TACATGG22750.034.8544582
TATCAAC33950.033.972482
GAATACG3200.033.747999
ATAAGTC14850.032.5233573
TAAGTCT16450.031.9892444
TATAAGT15750.031.5802522
GTATAAG17400.030.2464711
GGTATCA20400.029.3324971
GACTCGG6900.028.188017
CATTAGC6900.028.1739868
Produced by FastQC (version 0.11.5)